Maize Domestication and Anti-Herbivore Defences: Leaf-Specific Dynamics during Early Ontogeny of Maize and Its Wild Ancestors
Fig 2
Constitutive BX levels in multiple representatives of teosinte and cultivated maize.
Total levels of 1,4-benzoxazin-3-ones (BXs) at different growth stages of two teosinte accessions (T62 and T77), a Tuxpeño landrace variety from two collection sites (Talpitita and El Cuyotomate) and two maize hybrid lines (Pactol and Delprim) were calculated as the sum of seven individual BX glucosides and are given as μg / g fresh weight (FW) (mean ± SE; N = 3–5). At each growth stage old (blue) and young (red) leaves were sampled. Note that at L2 old and young leaf were the same. The specific positions of harvested leaves are highlighted in the maize drawings. Significant effects of plant genotype and leaf identity on total BXs are denoted by asterisks: * P<0.05; ** P <0.01; *** P <0.001; ns: not significant; two-way ANOVA without growth stage L2. Significant differences between plant lines are indicated by different letters and derive from Holm-Sidak tests for the respective leaf type. The concentrations of Ejutla and B73 from the previous figure are included for comparison (black bars).