The Immunoreactive Exo-1,3-β-Glucanase from the Pathogenic Oomycete Pythium insidiosum Is Temperature Regulated and Exhibits Glycoside Hydrolase Activity
Fig 10
Linear correlation of clear zone diameters and amounts of hydrolytic enzyme used in the agar plate enzymatic assay.
Laminarin-supplemented LB agar was hydrolyzed by various concentrations of cellulase enzyme (X-axis). Resulting clear zones (Y-axis) were visualized by staining with iodine. There is a linear relationship between the amount of enzyme and the diameter of clearing when displayed on a semi-log plot. Clear zone diameters generated by various pPinsEXO1-harboring E. coli cell suspensions (horizontal dash lines) were correlated with amounts of enzyme (vertical dash lines).