The Immunoreactive Exo-1,3-β-Glucanase from the Pathogenic Oomycete Pythium insidiosum Is Temperature Regulated and Exhibits Glycoside Hydrolase Activity
Fig 7
Peptide pre-absorption of the rabbit anti-Exo1 sera for Western blot analysis of P. insidiosum crude protein extracts.
Separated crude proteins (SABH) of P. insidiosum were probed with rabbit pre-immune serum (Lane 1), rabbit post-immune serum (Lane 2), and rabbit post-immune serum pre-absorbed with Peptide-A, -B, and -C (Lane 3), Peptide-A and -B (Lane 4), Peptide-A and -C (Lane 5), and Peptide-B and -C (Lane 6). The arrow and arrow head indicate the 82- and 78-kDa band, respectively. [Abbreviations: SABH, soluble antigen from broken hyphae; Pre-immune, rabbit pre-immune serum; Post-immune, rabbit anti-Exo1 peptide serum; ‘+’, used as probe (pre- and post-immune sera) or used for pre-absorption (Peptide-A, -B, or -C); ‘-’, not used as probe nor used for pre-absorption]