The Immunoreactive Exo-1,3-β-Glucanase from the Pathogenic Oomycete Pythium insidiosum Is Temperature Regulated and Exhibits Glycoside Hydrolase Activity
Fig 4
Expression of exo1 in response to temperature, culture duration, and dextrose availability.
Real-time PCR was used to measure exo1 mRNA levels in P. insidiosum (strain Pi-S) at 5 culture conditions: (i) 28°C for 7 days (28c-7d-1x); (ii) 37°C for 7 days (37c-7d-1x); (iii) 28°C for 14 days (28c-14d-1x); and 28°C for 7 days with (iv) 2 mg/ml dextrose (28c-7d-0.1x), or (v) no dextrose (28c-7d-0x) supplement. exo1 expression in each condition was normalized to the reference actin gene (act1). Asterisk indicates significant up- or down-regulation, relative to 28c-7d-1x.