Arginine Thiazolidine Carboxylate Stimulates Insulin Secretion through Production of Ca2+-Mobilizing Second Messengers NAADP and cADPR in Pancreatic Islets
Fig 5
nNOS plays a major role in ATC-induced Ca2+ signaling and insulin secretion in pancreatic β cell.
(A) Effect of nNOS inhibitor, ARL17477 (30 μM) and iNOS inhibitor, 1400W (100 μM) on ATC-induced Ca2+ signals. (B-D) Effect of nNOS and iNOS inhibitors on ATC-induced NO, cADPR, NAADP formation. (E) Effect of nNOS knock down (KD) on ATC-induced NO formation. (inset) Representative immunoblots for quantifications of nNOS protein expression in pancreatic β cell after infection with lentiviral particles expressing scrambled or nNOS-specific short hairpin (shRNA). (F) Effect of nNOS KD on ATC-induced Ca2+ signal. (G and H) Effects of nNOS KD on ATC-induced cADPR and NAADP formation. (I) Effect of nNOS inhibitors and nNOS KD on ATC-induced insulin secretion. *, P<0.05 versus CON level. #, P<0.05 versus ATC treated level. All data are expressed as the Mean ± SEM.