Synthesis and Evaluation of Chloramphenicol Homodimers: Molecular Target, Antimicrobial Activity, and Toxicity against Human Cells
Fig 8
Toxicity assays in Jurkat cells.
Jurkat cells were adjusted to 1×109 cells/L in RPMI-1640 medium containing 1% Penicillin/Streptomycin and 10% fetal bovine serum. The cells were grown in triplicate in the presence or absence of compound 5 at the indicated concentrations for 4 days at 37°C, under a humidified 5% CO2 atmosphere. CAM was used as a reference compound. For cell necrosis and apoptosis assays, samples (106 cells) were collected daily and determined by flow cytometry. Apoptotic and necrotic cells were expressed as a percentage of total cells.