Smooth Muscle Cell Genome Browser: Enabling the Identification of Novel Serum Response Factor Target Genes
Fig 5
Identification of SMC-specific genes by expression profiles and histone modifications.
(A) Specificity of SMC-enriched genes. Cell specificity was determined by comparative analysis of gene expression profiles among SMCs, ICCs, and PDGFRα+ cells: SMCsexpression level (FPKM)/[ICCexpression level (FPKM) + PDGFRα+ cellsexpression level (FPKM)] (B) Comparison of JSMC- and CSMC-enriched gene expression. (C) Genomic map views of histone modications (H3K4m3, H3K27a, and H3K27m3 in 8 week small intestine) on Cnn1 (specifically expressed in JSMCs and CSMCs) and Ubb (ubiquitiously expressed). (D) Comparison of histone modifications on SMC-enriched genes. The signal value is the average of the mininum and maxium values of the ChIP-seq signal for each histone modification. (E) Comparison of histone modifications on ubiquitious genes. (F) Expression levels of SMC-enriched genes not altered by histone modifications.