Inhibition of ANO1/TMEM16A Chloride Channel by Idebenone and Its Cytotoxicity to Cancer Cell Lines
Fig 5
Effects of ANO1 inhibition on the cell proliferation and apoptosis in PC3, CFPAC-1 and A549 cells.
(A-C) PC3, CFPAC-1 and A549 cells were seeded in 96 well plates, and after 24 h incubation, they were treated with the indicated concentrations of idebenone (IDE), 100 μM coenzyme Q10 (Q10) and 10 μM T16Ainh-A01 (A01) in MTS assay. IDE (30 μM), coenzyme Q10 (100 μM) and T16Ainh-A01 (10 μM) were applied to the cells in BrdU assay. Cell proliferation was estimated after 2 days via MTS (left) or BrdU (right) assay (mean ± S.E., n = 6). (D-F) PC3, CFPAC-1 and A549 cells were treated with 30 μM idebenone. The cells were stained with TUNEL (terminal deoxynucleotide transferase-mediated dUTP nick-end labeling, green) and DAPI (4,6-diamidino-2-phenylindole, blue). Scale bars represent 20 μm. *P < 0.05, Students’ unpaired t-test.