Autotaxin and Endotoxin-Induced Acute Lung Injury
Fig 6
Systemic overexpression of ATX exacerbates ALI.
Transgenic (Tga1t1Enpp2+/+) or heterozygous complete knock-out (Enpp2+/-) mice and their corresponding wild type littermate mice were administered with aerosolized LPS to induce lung injury and were sacrificed 24 hours later (n = 2–6; a representative experiment out of two is shown). A-D. Histological analysis (A) and BALF measurements (B & C) showed increased inflammation and edema in the lungs of the transgenic mice with systemic overexpression of ATX as a result of increased BALF ATX activity (D). On the contrary, histological analysis (A) and BALF measurements (E-G) showed non-significant effects in the lungs of the heterozygous knock-out mice as a result of decreased ATX activity levels.