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Counting White Blood Cells from a Blood Smear Using Fourier Ptychographic Microscopy

Fig 2

The full FOV image of a blood smear slide with SBP of ~0.9 gigapixel by FPM using 2x 0.08 NA objective (center) and its magnified subregions (surrounding).

The red subregions are directly extracted from the full FOV image, and the corresponding blue subregions are taken by a 20x 0.5 NA objective. They both offer enough details from which we can discern the shapes and sizes of the WBCs and their nuclei. The blue circle in the middle of the center image represents the relative size of the FOV achieved by a 20x objective compared to that of FPM.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0133489.g002