Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination
Fig 5
H2B monoubiquitination is involved in the DNA damage response.
a. Gene set enrichment analysis revealed that genes coding for components of the DNA damage repair were enriched following RNF20 knockdown and H2BK120R overexpression in HEK293T cells. b. Effects of RNF20 knockdown and H2BK120R overexpression on HR and NHEJ mediated DNA DSB repair. The DNA DSB repair efficiency was calculated as we previously described [27]. More than three replicates were used in each analyses. **, P < 0.01 (two-tailed unpaired t test; n > 3). c. H2BWT and H2BK120R mutant plasmids transfected HEK293T cells were subjected to X-Ray irradiation (80 Kv for 5 min), and then cells were cultured for the indicated times. Cells were harvested and analyzed by western blot with the specified antibodies.