Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath
Fig 5
The use of nanodroplets allowed an ultrasonic water bath to fragment genomic DNA.
(A) Schematic showing the ultrasonic bath used for sonication. Samples were immobilized in the water bath using a stand with a tube rack attached. The circulating water chiller was optional. Water chilled to four degrees Centigrade can be added just prior to sonication, with no loss in DNA fragmentation efficiency. (B) A time-titration was performed with samples with and without nanodroplets. Following fragmentation, samples were run on a 1.5% agarose gel and visualized using SYBR green. DNA ladder sizes are indicated in base pairs. (C) Arrangement of DNA samples fragmented in the ultrasonic bath with and without samples to produce (D) an acoustic field map of the bath. The fragmentation ability (base pair size) is visualized with the s1color bar, where red indicates complete fragmentation in the 200–500 bp range.