Yeast m6A Methylated mRNAs Are Enriched on Translating Ribosomes during Meiosis, and under Rapamycin Treatment
Fig 5
Sporulation phenotype under rapamycin treatment in normal and methylation deficient strains.
SK1 and ime4Δ/ ime4Δ strains were treated with rapamycin, and at different time points samples were checked for ascus formation. The sporulation pattern of SK1 cells under rapamycin treatment looked similar to sporulation under starvation conditions. Sporulation was completed by the 5th day with a 91±5% sporulation efficiency. The ime4Δ/ ime4Δ strain showed some level of sporulation (22.5±12%), but with abnormal ascus formation (a). After 5 days rapamycin treatment the ime4Δ/ ime4Δ cells mainly produced elongated cells with only a few producing asci which were all abnormal. In contrast, the SK1 cells maintained a high efficiency of sporulation, although diad and asymmetric asci, with a void volume were common (b).