Mutant IDH1 Dysregulates the Differentiation of Mesenchymal Stem Cells in Association with Gene-Specific Histone Modifications to Cartilage- and Bone-Related Genes
Fig 5
IDH1 R132C differentially regulated the expression of chondrocyte- and osteocyte-related genes by gene-specific epigenetic modulation.
Active and repressive histone marks associated with the promoter regions of the SOX9 (A), COL2A1 (B), and ALPL (C) genes. The status of histone modification in each hMSC (Par, EV, MT, or R132C, as described in the legend for Fig 1) was analyzed by chromatin immunoprecipitation (ChIP) using antibodies against H3K4me3, H3K9me3, and H3K27me3. The target region of each locus was shown in the scheme at the top of each graph. Data were presented by qPCR, and the values were indicated relative to the input. Error bars reflect SD in 3 experiments. TSS, transcription start site. **, p<0.01 by Dunnett`s multiple comparisons test compared to the parental cells.