Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish
Fig 1
The GBT-B4 genetic screen approach and results.
(A) Design features of the GBT-B4 gene trap and the nucleotide sequence of the 14XUAS used in the parental GBT-B1 and the 4.5xUAS syUAS engineered of GBT-B4. The consensus Gal4 binding site based on the 14XUAS is in red bold letters, sequences based on the Saccharomyces UAS are in bold green. The core Gal4 binding site CGGN11CCG is highlighted in yellow. The non-consensus nucleotide in the 4th binding site of the syUAS is in lower case (t instead of G). (B) Diagram of the genetic screen; arrows indicate the workflow. (C) Summary of the screen results.