Development of a Fluorescent Quenching Based High Throughput Assay to Screen for Calcineurin Inhibitors
Fig 4
The complete assay was automated and performed in a similar way mimicking all steps of the actual screening. 100% DMSO was used to replace the compound. (a) Two full 384 plates were used. Plate 1 and 2 yielded a Z score of 0.91 and 0.81; % CV 13.99 and 15.12 for the full reaction, respectively. (b) Z scores of 4 pilot plates screened in duplicate using this automated assay format.