Development of a Bioluminescent Nitroreductase Probe for Preclinical Imaging
Fig 2
Evaluation of NTR-specific uncaging of NCL probe.
(A) NCL (20 μM) uncaging by NTR (0.5 μg mL-1) in the presence of NADH (100 μM) was inhibited by dicoumarol (0 to 200 μM). (B) Total luminescence over 30 min from luciferin or NCL (0.25–5 μM) with NADH, luciferase, and NTR, compared with the control (no NTR), *P < 0.001.