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Diorcinol D Exerts Fungicidal Action against Candida albicans through Cytoplasm Membrane Destruction and ROS Accumulation

Fig 6

Effect of DD on the mitochondria function.

SC5314, preincubated with or without Tu or NaN3, was treated with various concentrations of DD and 8 mg/L of AMB (positive control) for 3 h before staining with 5 μM of Rh 123 to indicate the alteration of mitochondrial membrane potential (mtΔψ). The fluorescence intensity was analyzed by flow cytomerty and shown at (A). C. albicans CAI4-TOM70-GFP, preincubated with or without Tu or NaN3, was treated with various concentrations of DD. The cells were visualized by CLSM and shown at (B). The bars in (A) indicate standard deviations and in (B) indicate 5 μm.

Fig 6

doi: https://doi.org/10.1371/journal.pone.0128693.g006