Diorcinol D Exerts Fungicidal Action against Candida albicans through Cytoplasm Membrane Destruction and ROS Accumulation
Fig 5
Effect of DD on intracellular ROS production.
SC5314, preincubated with or without Tu or NaN3, was exposed to the increasing concentrations of DD or 8 mg/L of AMB (positive control) for 3 h. After staining with 40 mg/L of DCFH-DA, the samples were detected by flow cytometry and visualized by CLSM with 488 nm of excitation and 525 nm of emission. (A) showed the flow cytometry results and (B) showed the CLSM observation. The bars in (A) indicate standard deviations and in (B) indicate 5 μm.