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Diorcinol D Exerts Fungicidal Action against Candida albicans through Cytoplasm Membrane Destruction and ROS Accumulation

Fig 4

The ultrastructure of DD-treated C. albicans cells and the expressions of genes associated with cell membrane synthesis and cell wall assembly.

(A) and (B) showed the transmission electron micrographs of C. albicans with 12 mg/L of DD-treatment or not. The destruction and fragmentation of plasma membrane were observed in DD-treated cells indicated by arrow in B. (b) is the magnification of (a). (C) showed the effect of DD on the expression of cell membrane related genes. After treatment with 8 mg/L of DD for 3 h, SC5314 cells were harvested for the total RNAs extraction and then the expressions of indicated genes were measured using qPCR. Bars in (C) indicate standard deviations.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0128693.g004