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Diorcinol D Exerts Fungicidal Action against Candida albicans through Cytoplasm Membrane Destruction and ROS Accumulation

Fig 2

Effect of DD on the cell membrane integrity.

(A) showed the effect of DD on the cell membrane dynamic. SC5314 cells were treated with various doses of DD or 8 mg/L of AMB (positive control) for 3 h following with staining of DPH for spectrofluorophotometer detection. (B) showed the change of membrane permeabilization after cells were treated with DD or 8 mg/L of AMB (positive control). Treated cells were stained with PI and analyzed by flow cytometry. The cells with more than 101 fluorescence intensity were regarded as stained cells. Bars indicate standard deviations.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0128693.g002