The Frog Skin-Derived Antimicrobial Peptide Esculentin-1a(1-21)NH2 Promotes the Migration of Human HaCaT Keratinocytes in an EGF Receptor-Dependent Manner: A Novel Promoter of Human Skin Wound Healing?
Fig 5
Effect of mitomycin C (Mito) on the peptides-mediated closure of a pseudo-"wound" field produced in a HaCaT cells monolayer.
(A) After removal of the ibidi culture insert, HaCaT cell monolayers were pre-incubated or not with 3 μM Mito for 30 min and subsequently treated with 0.25 μM all-L Esc(1-21) or LL-37. Cells incubated with medium served as control (Ctrl). Samples were photographed at different time intervals, as indicated in the legends to Figs 2 and 4, and the percentage of cell-covered area was calculated and reported on the y-axis. All data are the mean of three independent experiments ± SEM. No statistical significance was found between samples treated with the peptide alone or pre-incubated with Mito.