Limb Ischemic Preconditioning Protects Endothelium from Oxidative Stress by Enhancing Nrf2 Translocation and Upregulating Expression of Antioxidases
Fig 2
Rat LIPC sera decreased ROS in H2O2-injured HUVECs.
ROS level was determined by measuring the intensity of dihydroethidium (DHE) fluorescence. The relative fluorescence intensities of DHE were analyzed by Image-Pro Plus software taking the fluorescence intensity of the control as 100%. A: original images of the cells preloaded with DHE. B: pooled data are expressed as mean ± SEM, n = 5, **P < 0.01 vs model.