Eriocalyxin B Inhibits STAT3 Signaling by Covalently Targeting STAT3 and Blocking Phosphorylation and Activation of STAT3
Fig 6
EB inhibited tumor cell growth by inducing apoptosis.
(A) MDA-MB-468, A549, MDA-MB-453 cells (3.0*105 per well) were treated with EB or Stattic at indicated concentrations for 2 h. Whole cell lysates were processed for western blot analyses using antibodies as indicated. (B) MDA-MB-468, A549, MDA-MB-453 cells were treated with EB or Stattic at indicated concentrations for 48 h. The viability of the three cell lines was analyzed by MTT assay. (C) A549 cells were treated with EB at indicated concentrations for 12 h. The whole cell lysates were processed for western blot analysis using antibody as indicated. (D) A549 cells were treated with EB (20 μM) for various time periods (0–48 h). Whole cell lysates were processed for western blot analysis using antibody as indicated.