Eriocalyxin B Inhibits STAT3 Signaling by Covalently Targeting STAT3 and Blocking Phosphorylation and Activation of STAT3
Fig 3
EB did not enhance the activity of tyrosine phosphatases.
(A) A549 cells were stimulated by IL-6 (10 ng/ml) for 15 min. The media were then replaced by fresh media without IL-6 and incubated with or without EB (20 μM) for different time periods (0–240 min). Whole cell lysates were processed for western blot analysis using antibodies as indicated. (B) A549 cells were pre-treated with sodium orthovanadate (50 μM), EB (20 μM) or their mixture for 2 h, then stimulated with IL-6 for 15 min. Whole cell lysates were processed for western blot analysis using antibodies as indicated.