Brown Pine Leaf Extract and Its Active Component Trans-Communic Acid Inhibit UVB-Induced MMP-1 Expression by Targeting PI3K
Fig 6
BPLE and TCA decrease collagen degradation and MMP-1 expression in a human skin equivalent model A, A schematic diagram of the 3D human skin cell culture system.
The experimental procedure was described in the Materials and Methods. B and C, BPLE and TCA inhibit UVB-induced MMP-1 protein expression levels and collagen degradation. The serial sections, from the human skin equivalent, were mounted onto silane-coated slides and subjected to Masson’s trichrome staining (B and D) or to immunohistochemical staining using anti-MMP-1 antibody (C and E). MMP-1 appears brown. Relative density was measured using the ImageJ program. Means with different letters (a-d) within a graph were significantly different from each other at p < 0.05.