Brown Pine Leaf Extract and Its Active Component Trans-Communic Acid Inhibit UVB-Induced MMP-1 Expression by Targeting PI3K
Fig 3
BPLE and TCA inhibit UVB-induced binding affinity of AP-1 residues in HaCaT cells.
A, B, and C, DNA binding activity of phospho-c-jun (A), c-Fos (B) and Fra-1 (C). Cells were treated with BPLE and TCA at the indicated concentrations for 1 h before being exposed to 0.01 J/cm2 of UVB and nuclear extract was collected 4 h later. DNA binding activity was measured by TransAM AP-1 DNA-binding ELISA kit. Means with different letters (a-d) within a graph were significantly different from each other at p < 0.05. D, Phosphorylated and total protein levels of c-Jun were measured by Western blot assay. Relative band intensity is expressed as fold of control and indicated on top of each band.