Brown Pine Leaf Extract and Its Active Component Trans-Communic Acid Inhibit UVB-Induced MMP-1 Expression by Targeting PI3K
Fig 2
BPLE and TCA inhibit UVB-induced MMP-1 mRNA expression and AP-1 transactivation in HaCaT cells.
A and B, Cells were treated with BPLE and TCA at the indicated concentrations for 1 h before being exposed to 0.01 J/cm2 of UVB and mRNA was collected 12 h later. MMP-1 mRNA expression was analyzed as qRT-PCR. C and D, BPLE and TCA attenuated UVB-induced AP-1 transactivation in HaCaT cells. Cells were treated with BPLE and TCA at the indicated concentration for 1 h before being exposed to 0.01 J/cm2 of UVB and cells were lysed 12 h later. AP-1 transactivation was measured using Luciferase assay. All data are presented as the mean ±S.D. determined from three independent experiments. Means with different letters (a-c) within a graph were significantly different from each other at p < 0.05.