Brown Pine Leaf Extract and Its Active Component Trans-Communic Acid Inhibit UVB-Induced MMP-1 Expression by Targeting PI3K
Fig 1
TCA contributes to the inhibitory effect of BPLE on UVB-induced MMP-1 expression in HaCaT cells.
A and C, Protein expression was analyzed by Western blotting (MMP-1), Zymography (MMP-2), and ELISA (MMP-1 contents). Cells were treated with BPLE/PLE (A) or TCA/DAA (C) at the indicated concentration for 1 h before being exposed to 0.01 J/cm2 of UVB, and media was harvested 48 h later. B and D, Cell viability of HaCaT cells in the presence or absence of BPLE, PLE (B) or TCA, DAA (D). Cell viability was measured using MTT assay. Each experiment was performed in triplicate. The data are presented as the mean ±S.D. of MMP-1 protein content and cell viability. Means with different letters (a-c) within a graph were significantly different from each other at p < 0.05.