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Allopregnanolone Preclinical Acute Pharmacokinetic and Pharmacodynamic Studies to Predict Tolerability and Efficacy for Alzheimer’s Disease

Fig 2

Mouse plasma and brain concentration-time profiles of allopregnanolone following intravenous (IV), subcutaneous (SC) and transdermal (TD) administration.

A. Dosing at 1.5 mg/kg IV using Allo solution in 6% HBCD vehicle in male non-transgenic mice (15-months-old, n = 5–6). B. Dosing at 10 mg/kg SC using Allo suspension in 5% EtOH/PBS vehicle in male non-transgenic mice (15-months-old, n = 1–3), at 10 mg/kg SC using Allo suspension in 5% EtOH/PBS vehicle in male 3xTgAD mice (5-months-old, n = 6) and 50 mg/kg TD using Allo solution 45% DMSO, 30% EtOH, 2.5% Klucel MF, 19.2% PEG-300 (5-months-old, n = 8). Data points represented as mean ± SD. LLOQ: Lower Limit of Quantification.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0128313.g002