Swertisin an Anti-Diabetic Compound Facilitate Islet Neogenesis from Pancreatic Stem/Progenitor Cells via p-38 MAP Kinase-SMAD Pathway: An In-Vitro and In-Vivo Study
Fig 1
PANC-1 cells differentiation and characterization with Activin-A.
(A) showed differentiation of Panc-1 cells subjected to differentiation using activin-A for 10 days. Figure shows bright field image of ILCC generated upon differentiation on day 10th at 10X magnification. Panels (A) showed Panc-1 ILCC immunostained on 10th day, positive for C-peptide, Insulin, and glucagon. Insulin was stained with TRITC labeled antibody showed red in color whereas c-peptide and glucagon were stained using FITC labeled antibodies showed green in color. (B) shows comparison of PANC-1 cells differentiation with Control SFM, Activin-A and Swertisin. Panc-1 cells cultured in complete media at day 0 which were then subjected to differentiation using SFM/ITS, Activin-A and Swertisin for 10 days. Bright field image of cells under differentiation for day 3rd, day 8th at 5X and 10X magnification respectively and dithizone stained ILCC on day 10th are shown. (C) shows comparative and qualitative insulin immunofluorescence signals in ILCC differentiated from SFM/ITS, Activin-A and Swertisin. Insulin is stained with Alexa-488 labeled antibody showed in red color and nucleus were counterstained with DAPI in blue. Pixel colocalization graphs shows co-localized distribution pattern of insulin and dapi fluorescence in differentiated ILCCs. (D) represents graph for quantification of insulin immunofluorescence per unit cytoplasm from immunostained ILCCs in Control SFM, Activin-A and Swertisin groups. Insulin signal stained with alexa-488 in Swertisin group compared to both SFM and activin-A group. Data was calculated from every single cell in three different frames per slide and expressed as mean ± SEM. *** and ** shows p value less than 0.001 and 0.01 in Swertisin group with respect to SFM and Activin-A group.