Interactions between Skeletal Muscle Myoblasts and their Extracellular Matrix Revealed by a Serum Free Culture System
Fig 5
ECM proteins support C2C12 myoblast proliferation in serum free cultures.
(A) Phase contrast images show C2C12 myoblasts cultured on collagen I (a, d), fibronectin (b, e) and solubilized muscle matrix (c, f) after 1 and 4 days in serum free culture. Scale bars are 150 μm. A section of “f” is shown at a higher magnification (Scale bar = 200 μm). Proliferation of C2C12 cells on protein substrates in serum free medium was assessed by a direct cell count (g). Data are mean ± SD of 4 independent experiments.