Comparative Evaluation of Sloppy Molecular Beacon and Dual-Labeled Probe Melting Temperature Assays to Identify Mutations in Mycobacterium tuberculosis Resulting in Rifampin, Fluoroquinolone and Aminoglycoside Resistance
Fig 3
Limit of detection (LOD) of the individual SMB and DLP assays on M. tuberculosis H37Rv genomic DNA.
Assays were performed with a dilution series of genomic equivalents as indicated. Assay success rates for individual probes and the entire assay over the input DNA dynamic range are shown. Panel (A) SMB probe and assay performance; Panel (B) DLP probe and assay performance.