Galectin-3 Overrides PTRF/Cavin-1 Reduction of PC3 Prostate Cancer Cell Migration
Fig 5
Exogenous Gal3 does not restore FAK stabilization in FAs of Cav1 knockdown cells.
(A) Western blot shows the efficiency of Cav1 siRNA knockdown. (B) FRAP assay of FAK-GFP shows FA-associated FAK stability of PC3 cells transfected with no siRNA, scramble control siRNA (siCTL) or the siRNA against human Cav1 (siCav1), and subjected to 2 μg/ml Gal3-His treatment. The FAK-GFP intensity recovery curve graphs of one representative experiment and a bar graph of the FAK-GFP mobile fraction summarized from all experiments are shown. (n = 3; ***: p<0.001).