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Galectin-3 Overrides PTRF/Cavin-1 Reduction of PC3 Prostate Cancer Cell Migration

Fig 4

Exogenous Gal3 restores FAK stabilization in FAs of Gal3 knockdown cells.

(A) Western blot shows the efficiency of Gal3 siRNA knockdown. (B) FRAP assay shows FA-associated FAK-GFP stability in PC3 cells transfected with no siRNA, scramble control siRNA (siCTL) or the siRNA against human Gal3 (siGal3), and subjected to 2 μg/ml Gal3-His treatment. The FAK-GFP intensity recovery curve graphs of one representative experiment and a bar graph of the FAK-GFP mobile fraction summarized from all experiments are shown. (n = 3; ***: p<0.001.)

Fig 4

doi: https://doi.org/10.1371/journal.pone.0126056.g004