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Branchial Cilia and Sperm Flagella Recruit Distinct Axonemal Components

Fig 5

Predominant localization of tektin 3 to branchial cilia.

Immunofluorescent microscopy was carried out on trimmed brancial wall with anti-Tctex2 dynein light chain antibody (middle) and anti-tektin 3 antibody (bottom). Non-immune anti-serum was used as a control (top). Samples were stained with anti-acetylated α-tubulin (green), anti-Tctex2 or anti-tektin 3 (red), or 4',6-diamidino-2-phenylindole (DAPI; DNA, blue). Left, double staining with DAPI and anti-acetylated α-tubulin; center, double staining with anti-Tctex2 or anti-tektin 3 antibody; right, merged image from DNA staining, staining with anti-acetylated tubulin antibody and staining with anti-Tctex2 or—tektin 3. Bar, 20 μm.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0126005.g005