Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion
Fig 5
Rab27A exhibited different subcellular localization than Rab27B and Rab3D.
(A) Cell fractionation samples from ICR mouse pancreas were loaded at 10 μg/lane and separated by PAGE. Antibodies against small G-proteins and marker proteins of different subcellular compartments were used in western blots. (B) Densitometry analysis on the western blot results from purified ZG membrane, mitochondrial and microsomal fractions shown in (A). The results are mean ± SE from three separate cell fractionation experiments. Pancreas tissues from 5–6 ICR mice were used in each experiment. *P < 0.05.