(2-Hydroxypropyl)-β-Cyclodextrin Is a New Angiogenic Molecule for Therapeutic Angiogenesis
Fig 7
Effect of L-NAME on 2HP-β-CD induced stimulation of blood flow recovery in C57BL/6 mice.
A, Ischemic/non-ischemic limb blood flow ratio assessed by LDBF imager in mice in 4 experimental groups; PBS-treated mice with or without L-NAME, 2HP-β-CD-treated mice with or without L-NAME. Data represent the mean ± S.E.M. (n = 10 mice per group). * p < 0.05, 2HP-β-CD-treated mice versus PBS-treated mice. § p < 0.05, 2HP-β-CD-treated mice versus 2HP-β-CD with L-NAME-treated mice; ＃ p < 0.05, PBS with L-NAME-treated mice versus 2HP-β-CD with L-NAME-treated mice. B, Immunohistochemical analysis of CD31 in ischemic hindlimbs of PBS-treated and 2HP-β-CD-treated mice with or without L-NAME. Representative images (upper panel) from calf muscle on postoperative day 28 are shown. Quantitative analysis of CD31-positive microvessel density is also shown (lower panel). Data represent the mean ± S.E.M. (n = 5 mice per group). * p < 0.05. Scale bar = 100 μm.