The Use of Maleic Hydrazide for Effective Hybridization of Setaria viridis
Fig 5
The effect of maleic hydrazide (MH) on the pre-anthesis anthers of Setaria.
(A) Untreated control spikelet and (B) spikelet treated with 500 μM MH. (C and D) Androecium and gynoecium of control and MH-treated spikelets, respectively. (E and F) Anthers of control and MH-treated spikelets, respectively. (G and H) A representative anther of control and MH-treated spikelets, respectively. (I) In control panicles, mature seeds were observed in the spikelets. (J) Brown specks were observed as remnants of anther dehiscence in the MH-treated panicles and no seeds were set although the florets remained green. The scale bar represents 5 mm in A and B, 0.5 mm in C, D, E and F, and 0.75 mm in G and H; I and J are not to scale.