The Use of Maleic Hydrazide for Effective Hybridization of Setaria viridis
Fig 1
Emasculation of Setaria viridis.
(A) Panicles that had just emerged completely out of the flag leaf sheath, such that the lowest spikelets of the panicles were between 0 and 2 cm from the collar of the flag leaf were selected. (B) The spikelets from tip and base of the panicle were trimmed; leaving approximately 50 florets and the bristles were also trimmed. (C) The panicles were completely immersed in the MH solution for 2 min, and excess liquid was gently wiped using tissue paper and (D) then the plant was left to stand until the panicles were dry. (E) After wiping off the solution and drying, the panicles were placed in glassine bags of appropriate sizes. (F) The glassine bag was labeled with the plant designation and emasculation date. Steps D and E were repeated on the same panicle for three consecutive mornings. (G) Panicle, 10 days after pollination.