Sutureless Fixation of Amniotic Membrane for Therapy of Ocular Surface Disorders
Fig 4
Morphology of AMs after therapeutic application.
A) AM appears condensed at the site of the ring; B) AM without epithelium (patient #3 second application); C) some AMs retained their own monolayered epithelium (patient #5 first application L); D) same AM as in C, showing a double-layered epithelium with larger cells at the stromal side which faced the patient eye during application; E) some AMs had a multi-layered stratified epithelium with big, cuboidal cells with large round nuclei on the epithelial side which faced the air during application (patient #3 first application); F) same AM as in E, here the stratified epithelium at the epithelial side appeared bulky and the cells were of irregular size. HE staining; es: epithelial side; ss: stromal side; scale bar in A 100 μm, scale bar in B-F 50 μm.