STAT3 Decoy Oligodeoxynucleotides-Loaded Solid Lipid Nanoparticles Induce Cell Death and Inhibit Invasion in Ovarian Cancer Cells
Fig 3
Uptake of SLN-decoy ODN complexes in ovarian cancer cells.
(A) Fluorescent micrographs of SKOV3 and A2780 cells transfected with naked decoy ODN, SLN-decoy ODN and Lipofectamine 2000-decoy ODN at a concentration of 25 nmol/L at 24 and 48 hours post transfection. (B) Flow cytometry analysis of cellular uptake of SKOV3 and A2780 cells transfected with SLN-decoy ODN complexes at concentrations of 0, 12.5, 25 and 50 nmol/L at 48 hours post transfection. (C) Flow cytometry analysis of cellular uptake of SKOV3 and A2780 cells transfected with naked decoy ODN, SLN-decoy ODN and Lipofectamine 2000-decoy ODN complexes at a concentration of 25 nmol/L at 48 hours post transfection. (D) and (E) Western blot analysis of STAT3 and p-STAT3 (Ser727, Tyr705) protein expression. The relative protein levels were expressed as the ratio of protein of interest/β-actin. The data are expressed as the means ± SD of three independent experiments, ** P < 0.01, # P > 0.05.