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Cambinol, a Novel Inhibitor of Neutral Sphingomyelinase 2 Shows Neuroprotective Properties

Fig 8

Effect of cambinol on TNF-α or IL-1β-induced loss of viability in neurons.

(A) Effects of cambinol (0.1–25 μM), inactive analog compound 2 (10–25 μM) or zoledronic acid (10–25 μM) on the survival of rat primary neurons treated with TNF-α or IL-1β. (100 ng/ml for 18 h) (B) Effects of SIRT1/2 inhibitors CHIC-35 (200 nM) and sirtinol (40 μM) on TNF-α induced neuronal cell death. Compounds were added 15 min prior to cytokine. Error bars correspond to S.E.M. of at least 3 independent determinations. *** p < 0.001.

Fig 8

doi: https://doi.org/10.1371/journal.pone.0124481.g008