Cambinol, a Novel Inhibitor of Neutral Sphingomyelinase 2 Shows Neuroprotective Properties
Fig 7
Effect of cambinol on TNF-α-induced changes in ceramide profiles of rat primary neurons.
(A) Heat-map represents ceramide profiles of rat primary neurons after treatment with TNF-α, cambinol or cambinol + TNF-α. Columns represent control, 100 ng/ml TNF-α for 2 min, 10 μM cambinol and cambinol + TNF-α. Drug was added to cells 15 min prior to TNF-α stimulation. Heat maps were generated based on normalization of the ceramide values using z transformations. Color scale illustrates quantitation, red color indicates increase of ceramide abundance and green color indicates depletion with respect to control treatment. (B) Quantitative analysis representation of ceramide levels for the four treatments shown in (A).