Cambinol, a Novel Inhibitor of Neutral Sphingomyelinase 2 Shows Neuroprotective Properties
Fig 5
Inhibition of human nSMase2 by cambinol and closely related analogs.
(A) Cambinol (compound 1) structure. (B) Dose response curve for inhibition of human nSMase2 by increasing concentrations of cambinol ([14C]-SM assay). (C) Non-linear least squares fit of the Michaelis-Menten equation for the activity of human nSMase2 vs. SM concentration in the presence of different concentrations of cambinol. Km and Vmax values decrease with increasing concentrations of inhibitor. (D) Lineweaver-Burk plot for data shown in (C). (E) Dose response curve for inhibition of human nSMase2 by cambinol analog compound 2. (F) Dose response curve for inhibition of human nSMase2 by cambinol analog compound 6.