Cambinol, a Novel Inhibitor of Neutral Sphingomyelinase 2 Shows Neuroprotective Properties
Fig 4
Characterization of fluorescence based activity assay for human nSMase2.
Plots show the dependence of enzymatic activity with respect to (A) protein concentration in the presence of 250 μM SM for 1 h; (B) substrate concentration for 0.6 μg of protein from human nSMase2-containing cell lysate for 1 h; and (C) time in the presence of 0.6 μg of protein from nSMase2 expressing cell lysate and 250 μM SM. Data for activity vs. substrate concentration were fitted by non-linear least squares fitting to the Michaelis-Menten equation to determine Km and Vmax.