Cambinol, a Novel Inhibitor of Neutral Sphingomyelinase 2 Shows Neuroprotective Properties
Fig 1
Fluorescence and radioactivity assays for human nSMase2.
(A) nSMase2-catalyzed reaction in the direct confirmatory assay with 14C-labeled SM (labeled atoms shown in magenta), which is hydrolyzed to generate labeled product. (B) Fluorescence assay for human nSMase2 consisting of three coupled reactions (in blue) and Amplex red dye to generate a red fluorescent product, resorufin (in red).