Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic
Fig 6
Effect of NaCl concentration on peptide loss in 220 μ L 2 μ M solutions.
(A) Percentage of recovered peptide for 200 μL 2 μM standard solutions prepared in 10 mM HEPES buffers of varying NaCl concentration directly in limited volume inserts. (B, C, and D) Percentage of recovered peptide for 220 μL 2 μM mastoparan X (B), melittin (C), and magainin 2 (D) solutions incubated in 10 mM HEPES buffers of varying NaCl concentration for 1 h in borosilicate glass vials, polypropylene tubes, or Protein LoBind tubes. The data are the average of two separate experiments, except in (A) in which three experiments are averaged. The error bars show the standard deviations. The percentage of recovered peptide was not strongly influenced by the NaCl concentration.