Characterization of Binding and Inhibitory Properties of TAK-063, a Novel Phosphodiesterase 10A Inhibitor
Fig 1
In vitro autoradiography (ARG) using [3H]TAK-063 in sagittal rat brain sections.
The chemical structure of [3H]TAK-063 (A). Sections adjacent to those used for in vitro ARG of [3H]TAK-063, were stained with hematoxylin and eosin (B). The autoradiogram shows the high accumulation of [3H]TAK-063 in the caudate putamen (CPu; white arrow), nucleus accumbens (NAc; black arrow), globus pallidus (GP; white arrow head), substantia nigra (SN; black arrow head), and striatonigral projection (gray arrow; C). In vitro ARGs in the presence of an excess amount of MP-10 (D) or TAK-063 (E) were performed with adjacent sections. Radioactivity levels in several brain regions were represented as photostimulated luminescence (PSL) values in the presence or absence of an excess amount of MP-10 or TAK-063 (F). Statistical analyses were performed using Dunnett's test (*P ≤ 0.05, **P ≤ 0.01 vs total binding, n = 3). Fcx, frontal cortex; Thal, thalamus; Bs, brainstem; Hipp, hippocampus; Cb, cerebellum.