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Characterization of Dedifferentiating Human Mature Adipocytes from the Visceral and Subcutaneous Fat Compartments: Fibroblast-Activation Protein Alpha and Dipeptidyl Peptidase 4 as Major Components of Matrix Remodeling

Fig 1

Dedifferentiating human mature adipocytes.

A) Confocal microscopy of human mature adipocytes undergoing dedifferentiation at various stages (10x) B) Oil red O staining of OM mature adipocytes undergoing dedifferentiation in ceiling culture (4x). Days 4, 7 and 12 are shown. C) Lipid droplet size measurement of dedifferentiating mature adipocyte cells at days 4, 7 and 12 (n = 6, 4M; 2F, BMI range: 39–56.9 kg/m2, Age range: 29–59 years). Left panel: Statistical MANOVA analysis: Time p<0.05. Middle and right panels: Student T-tests *p<0.05.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0122065.g001